high productive infection against generic e coli atcc 13706  (ATCC)

Journal: Frontiers in Microbiology

Article Title: Penetrating the biofilm barrier: characterization of Escherichia phage vB_EcoS-TPF103dw and harnessing depolymerase to combat Shiga toxin-producing Escherichia coli O103 biofilm

doi: 10.3389/fmicb.2025.1715907

Figure Lengend Snippet: Circular genome map of E. coli phage vB_EcoS-TPF103dw (length = 113,080 bp), annotated using Prokka and Bakta pipelines via Proksee. Predicted coding sequences (CDS), structural RNAs (tRNA/rRNA), and open reading frames (ORFs) are represented as colored blocks (red, blue, and green, respectively) across concentric rings. Functional annotations include structural phage components, lytic enzymes (e.g., endolysins, holins), and putative host-interaction proteins. Notable features include the terminase large subunit, tail fiber proteins, and chaperones indicative of complex host recognition mechanisms.

Article Snippet: In contrast, generic E. coli ATCC 13706, the isolation host, produced significantly larger plaques than O103 strains, while some O103 isolates formed pin-sized plaques that complicated titer enumeration ( ).

Techniques: Functional Assay

Journal: Frontiers in Microbiology

Article Title: Penetrating the biofilm barrier: characterization of Escherichia phage vB_EcoS-TPF103dw and harnessing depolymerase to combat Shiga toxin-producing Escherichia coli O103 biofilm

doi: 10.3389/fmicb.2025.1715907

Figure Lengend Snippet: Stability of phage: biological characteristics of phage TPF103dw, including various pH stability at 25°C for 24 h (A) , temperature stability for 24 h (B) , and a one-step growth curve of generic E. coli ATCC 13706 (C) . Error bars indicate 95% confidence intervals for pH and temperature, and standard error bars for one-step growth curve. Asteriks indicated statistically significant differences among groups and p < 0.05.

Article Snippet: In contrast, generic E. coli ATCC 13706, the isolation host, produced significantly larger plaques than O103 strains, while some O103 isolates formed pin-sized plaques that complicated titer enumeration ( ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Penetrating the biofilm barrier: characterization of Escherichia phage vB_EcoS-TPF103dw and harnessing depolymerase to combat Shiga toxin-producing Escherichia coli O103 biofilm

doi: 10.3389/fmicb.2025.1715907

Figure Lengend Snippet: Bacterial challenge assay for E. coli O103, five E. coli O103 treated with phage TPF103dw at MOI 10,000 at 25 °C for 24 h based on the bacterial optical density at wavelength 600 nm (OD 600 ). The control group only contains bacterial culture.

Article Snippet: In contrast, generic E. coli ATCC 13706, the isolation host, produced significantly larger plaques than O103 strains, while some O103 isolates formed pin-sized plaques that complicated titer enumeration ( ).

Techniques: Control

Journal: Frontiers in Microbiology

Article Title: Penetrating the biofilm barrier: characterization of Escherichia phage vB_EcoS-TPF103dw and harnessing depolymerase to combat Shiga toxin-producing Escherichia coli O103 biofilm

doi: 10.3389/fmicb.2025.1715907

Figure Lengend Snippet: Scanning electron micrographs of E. coli O103 biofilms on stainless steel surfaces. Planktonic E. coli O103 cells were allowed to settle on sterile stainless steel coupons and visualized using SEM at 5,000 × magnification (A) , Day 2 untreated control biofilm at 800 × magnification, showing a thick, dense matrix with extensive extracellular material characteristic of mature biofilm (B) . Corresponding phage-treated biofilm at 800 × magnification, exhibiting visibly reduced biomass and a disrupted matrix structure (C) . Higher magnification (10,000×) view of the control biofilm, revealing dense cellular packing and abundant extracellular polymeric substances (EPS) (D) . Phage-treated biofilm at 10,000 × magnification, displaying isolated bacterial cells with visibly reduced EPS and disrupted aggregation (E) . Biofilm treated filtrate containing soluble phage-derived enzymes (850 × magnification) reveals compact cell stacking with a complete absence of visible EPS (F) . Biofilm treated with filtrate containing soluble phage-derived enzymes (10,000 × magnification) reveals compact cell stacking with a complete absence of visible EPS (G) . These images highlight phage-derived enzymatic activity that may degrade biofilm matrix, supporting potential anti-biofilm mechanisms. Images demonstrate the structural impact of phage treatment on biofilm integrity.

Article Snippet: In contrast, generic E. coli ATCC 13706, the isolation host, produced significantly larger plaques than O103 strains, while some O103 isolates formed pin-sized plaques that complicated titer enumeration ( ).

Techniques: Sterility, Control, Isolation, Derivative Assay, Activity Assay

Journal: Microbiology Spectrum

Article Title: Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

doi: 10.1128/spectrum.02220-21

Figure Lengend Snippet: One-step growth curve of phage Sa45lw using generic E. coli strain (ATCC 13706). The growth parameters of the phage indicate an eclipse period (EP) of 17 min, a latent period (LP) of 27 min, and average burst size (BS) of 80 phages per infected cell. Closed red triangles indicate non-chloroform-treated samples; closed blue circles indicate chloroform-treated samples. The error bars present the standard error of the mean (SEM) for each time point of the one-step growth curve.

Article Snippet: The results of growth factors indicated that phage Sa45lw had an estimated 17-min eclipse period and a 27-min latent period against generic E. coli ATCC 13706 strain ( ).

Techniques: Infection

Journal: Microbiology Spectrum

Article Title: Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

doi: 10.1128/spectrum.02220-21

Figure Lengend Snippet: Host range and efficiency of plating (EOP) of phage Sa45lw against various Shiga toxin-producing Escherichia coli (STEC) and Salmonella enterica strains

Article Snippet: The results of growth factors indicated that phage Sa45lw had an estimated 17-min eclipse period and a 27-min latent period against generic E. coli ATCC 13706 strain ( ).

Techniques:

Journal: Microbiology Spectrum

Article Title: Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

doi: 10.1128/spectrum.02220-21

Figure Lengend Snippet: Antimicrobial activities of phage Sa45lw against (A) E. coli O45:H16 (RM13752) and (B) E. coli O157:H7 (RM35150) at an MOI of 10 in LB at 25°C for 24 h. The control group contained bacterial culture without phages (blue solid line), and the treatment group contained bacterial culture treated with phage Sa45lw (green dashed line). Data of each time point (0, 2, 4, 6 or 24 h) were analyzed separately, and asterisk indicates a significant difference at P < 0.05 between the control and treatment groups. The error bars present the SEM for each time point of the control and treatment.

Article Snippet: The results of growth factors indicated that phage Sa45lw had an estimated 17-min eclipse period and a 27-min latent period against generic E. coli ATCC 13706 strain ( ).

Techniques: Control

Journal: Microbiology Spectrum

Article Title: Characterization of a T4-like Bacteriophage vB_EcoM-Sa45lw as a Potential Biocontrol Agent for Shiga Toxin-Producing Escherichia coli O45 Contaminated on Mung Bean Seeds

doi: 10.1128/spectrum.02220-21

Figure Lengend Snippet: Application of phage Sa45lw on the mung bean seeds contaminated with 4.8 log CFU/g of E. coli O45:H16 (RM13752) at an MOI of 1,000 for 15 min. Treated mung bean seeds were stored at room temperature (25°C) for 24 h. Asterisk indicates a significant difference at P < 0.05 between the time point (6 or 24 h) and the initial treatment (0 h). The error bars present the SEM for each time point.

Article Snippet: The results of growth factors indicated that phage Sa45lw had an estimated 17-min eclipse period and a 27-min latent period against generic E. coli ATCC 13706 strain ( ).

Techniques: